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Based on previous experience, the diagnostic work-up should include the following: careful history taking, clinical manifestation, laboratory examinations such as liver function tests, serum ceruloplasmin, 24 h urinary excretion of copper, ophthalmologic examination, liver biopsy, measurement of the hepatic parenchymal copper level, hepatic imaging studies, and genetic testing [72]

For this interaction to occur, the structural requirement in the enzyme is the presence of a nucleophilic amino acid side-chain close to the glutathione-docking motif and in a crucial position to interact with the electrophilic motif of glutathione acrylate, as shown in Scheme 9

A certain amount of oxidative stress is actually a normal part of pregnancy

However, desiccants represent a secondary measure and cannot substitute for appropriate temperature control