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In site-specific incorporation, a codon is reprogrammed to encode a ncAA through a variety of techniques such as chemical modification of the acylated amino acid on the tRNA, 460462 chemoenzymatic ligation of an acylated adenosine onto a 3 truncated tRNA, 463465 enzymatic acylation using aaRSs, 466 and flexizyme-mediated methods

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The implications for training of embracing: a life course approach to health