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The slides were conjugated with primary antibodies for 30 min at room temperature, followed by antibody detection using Dako REAL Envision detection system (Agilent Dako, Carpinteria, CA, USA)

A one-way valve separates the chamber from the buffer below

The pEGFP-puro reporter vector was also utilized as a control vector in the transfection procedure

10.3390/biomedicines9050526 [DOI] [PMC free article] [PubMed] [Google Scholar] Raghunandan M, Yeo JE, Walter R, Saito K, Harvey AJ, Ittershagen S, Lee E, Yang J, Hoatlin ME, Bielinsky AK, Hendrickson EA, Schrer O, Sobeck A (2020) Functional cross talk between the Fanconi anemia and ATRX/DAXX histone chaperone pathways promotes replication fork recovery