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The cells were harvested and lysed in IP lysis buffer containing protease and phosphatase inhibitors

In the oxidation mode, the one-electron reduction of quinones generates the highly reactive semiquinone radical leading to generation of superoxide anions (Figure 2)
This study also found that cannabinoid receptors show a decrease during NE differentiation, and cannabinoid receptors have a tonic inhibitory effect on NE differentiation (Morell et al

Nat Rev Dis Primers 7(1):47
