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Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads Adding interacting proteins Eluting the beads Analyzing pull-down results What is a GST pull-down assay

Uncontrolled free radicals activity can lead to oxidative stresses, which have been implicated in breakdown of vital biochemical compounds such as lipids, protein, DNA which may lead to diabetes, accelerated ageing, carcinogenesis and cardiovascular diseases

Here, the authors describe the current landscape of genetic therapies for ALS and discuss new opportunities for gene replacement therapy, focusing on loss-of-function mutations.