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An aliquot of the sample (500 L) was mixed with 100 L of 6 N HCl and 600 L of benzene and stirred for 5 min

Gln, a nonessential amino acid abundant in the bloodstream, along with its metabolites lutamate and ketoglutarate (-KG), serves as a vital source of carbon and nitrogen, providing essential raw materials for the synthesis of nucleotides and amino acids ( Although some Gln metabolic inhibitors have entered clinical trials, the heterogeneity among tumors complicates their effectiveness