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Co-immunoprecipitation (Co-IP) assay Cells after indicated treatments were lysed in IP buffer (50 mM Tris-HCl, pH 7.5, 0.5% NP-40 (MF430, Mei5bio), 150 mM NaCl, 5 mM EDTA, 10% Glycerin) with protease inhibitor cocktail (B14001, Bimake) for 30 min at 4 C, followed by the enrichment of target proteins using Flag-M2 beads (B23102, Bimake) or specific antibodies overnight at 4 C

MountjoyMAckermanKBaileyDBurkeLConstantiniNHackneyAet al

It is the only enzyme in the GPX family that directly reduces and destroys lipid hydroperoxides

Yes, adults of all ages can benefit, especially those 2050 years old, dealing with pigmentation or skin dullness