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Real-time polymerase chain reaction (PCR) was performed by using a kit (SYBR Premix EX Taq, Takara Bio Inc.) and the ABI PRISM 7300 real-time PCR system

The needle may be moved around the trigger point to reduce the spasm before injecting medication

Noto H, Goto A, Tsujimoto T, et al

[DOI] [PMC free article] [PubMed] [Google Scholar] 107.Montero AJ, Jassem J
