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Western Blotting and antibodies Cultured cells were washed, dissociated using trypsin (Gibco, 15400-054), and pelleted prior to being snap-frozen in liquid nitrogen

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As an effector molecule, NO participates in antimicrobial responses and cooperates with oxidative stress processes to amplify inflammatory signaling, thereby being associated with an M1-like pro-inflammatory program ( + macrophage-like state with immunoregulatory features in mouse macrophage models ( In addition to generating terminal effector molecules, Arg metabolism can also influence inflammatory transcriptional programs through metabolic-signaling coupling