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It is known that a widespread characteristic of tumors is their inability to develop adequate blood vessels with the consequence being relative hypoxia: at moderate levels, ROS induce transcription of HIF1, the founding member of the family of hypoxia-induced factors, and stabilize the encoded protein, which is normally hydroxylated within less than 5 min, by inhibiting the activity of the iron-dependent prolyl 4-hydroxylase (PHD) involved in its degradation 29

The primary antibodies (Nrf2 [#23832], Keap1 [#4678], HO-1 [#5853]) were reacted at 4 C for 16 h, and the secondary antibody (anti-rabbit IgG, #7074) was reacted at 25 C for 2 h

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