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Neuroradiology 32 , 211214 (1990)

Difficulties found in the binding of MBP fusion proteins to amylose resins corroborate the hypothesis that target proteins interact with MBP via its binding site ( Other affinity tags, specific proteases and protein cultivation strategies are being employed together with MBP to improve protein soluble production, purification and native protein recovery, as for instance, His 6 -MBP fusions (Nallamsetty et al., 2005), His 6 -MBP-TEV fusions (Rocco et al., 2008), MBP-His 6 -Smt3 fusions in which the Saccharomyces cerevisiae Smt3 protein is used for protein processing by proteolytic cleavage between the MBP-His 6 tags and the protein of interest (Motejadded and Altenbuchner, 2009), and secretion of MBP fusion protein into the culture medium (Sommer et al., 2009)

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