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Then the cells were covered with 150 L of 1% CV solution (Sigma) and incubated for 45 min at room temperature
Tip: If you see dripping, re-seat the rubber washer/O-ring and tighten gentlysnug is enough
Because kinases generally phosphorylate a spectrum of substrate proteins, it is unlikely that ISR kinases only phosphorylate eIF2
J Ovarian Res (2021) 14(1):13
