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After dewaxing, rehydration and antigen retrieval according to the aforementioned method, the sample was endogenous peroxidase activity was quenched using 3% hydrogen peroxide solution and the sections were blocked using 5% bovine serum albumin (Roche Diagnostics GmbH) for 10 min at room temperature

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For example, chelating agents may induce toxicity by binding with other metal ions while off-target effects make copper regulation using copper ion carriers difficult, thus additional studies on how to regulate copper levels are required (180)

Science 304 , 16781682 (2004)