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Fact: Only supplements with significant calories, sugars, or specific amino acids (like BCAAs) trigger an insulin response large enough to break a metabolic fast

Ser is a substrate for mitochondrial onecarbon metabolism, which generates NADPH while converting Ser + tetrahydrofolate (THF) into glycine (Gly) + THF + formate through the activity of serine hydroxymethyl transferase 2 (SHMT2), methyleneTHF dehydrogenase (MTHFD) 2, and MTHFD1L

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However, the GK rat kidneys did not show a significant change in activity, though increased activities were observed after exercise in both the control Wistar and GK rats