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Lutein and Zeaxanthin are the optical pigments essential to the healthy function and protection of the retina and its macular region

Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36

After 24 h, bilirubin (0.1 M) was added into the cells, then after 6 h, the cells were treated with or without doxorubicin (1 M) for 24 h, collected the cells and washed with cold PBS and then resuspend cells in 1 Binding Buffer, and then transfer 100 l of the solution (1 10 5 cells) to a 2 ml culture tube, and add 5 l of FITC Annexin V and 5 l PI, and gently vortex the cells and incubate for 15 min at 25C in the dark room, and finally add 400 l of Binding Buffer to each tube, and then analyze it by flow cytometry