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A 24 h time point was chosen based on a previous report of using this time period for transfecting or treating 3T3-L1 cells with NNMT anti-sense oligonucleotides or a small molecule NNMT product inhibitor (1-MNA), respectively, for phenotypic measures.[12] MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (ATCC
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Biological age can be influenced by epigenetic factors, where lifestyle and interventions like NAD+ therapy can effectively "turn back the clock" at a molecular level

Side effects may include gastrointestinal upset, headache, or changes in appetite
