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After completing respirometry analyses, muscle bundles were dried in a lyophilizer (FreeZone 2.5 L, Labconco, Kansas City, MO) and weighed with a microbalance (MX5, Mettler Toledo, Columbus, OH)

These lipids are essential for maintaining exosomal structural integrity and functionality, particularly in their role as drug-delivery vehicles [75]

After that, 2 mL suspension was removed and added to fresh 100 mL MSM with DEHP (200 mg/L)

The defects arise from failure of the embryonic neural tube to close, which occurs between the 21st and 28th days after conception, a time when most women are unaware of their pregnancy (57)