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It also cleans up any heavy metals and regulates the body's nitric-oxide cycle, which is vital to homeostasis
After overnight incubation at 4 C with respective primary antibodies, cells were incubated with either Cy3-conjugated (red) or FITC-conjugated (green) secondary antibodies for 1 h at room temperature the following day
Advansta, GRISP), according to the supplier's instructions, and images were recorded using a Molecular Imager Gel Doc XR + System (Bio-Rad) and analyzed with ImageLab (version 5.0, Bio-Rad) (Westermann 2010)
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