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Library preparation and RNA sequencing (RNA-Seq) For genome-wide analysis of gene expression, RNA sequencing libraries from isolated mRNA were generated and sequenced by the Institute for Lung Health (ILH) Genomics and Bioinformatics at the Justus-Liebig-University (JLU) Giessen (Germany)
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GST tagged fusion proteins bind to glutathione agarose (column 2) and, after washing, are eluted with elution buffer with excess glutathione (column 3)
