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Activation of KORs (expressed in the spinal dorsal horn) can block the release of excitatory neurotransmitters, including glutamate and substance P, which in turn attenuates nociceptive signaling [62]

After centrifugation at 3000 g for 10 min, the supernatants were evaporated in a vacuum centrifugal evaporator EC-57CS (Sakuma, Tokyo, Japan) and dissolved in 1% (v/v) acetic acid

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These mutant mice displayed constitutively active NF-B, which led to a prominent increase in macrophages/microglia, T cells, and key proinflammatory mediators (45)