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N., and Basbaum, A

Cells cultured in triplicate at 50-70% confluency were transfected with specific pooled siRNA Oligo Duplex (Origene Technologies, Rockville, MD, USA) or control siRNA Duplex

The point is to match the treatment to the condition, not to climb a ladder for the sake of climbing it

Furthermore, TNF- affects the expression of the GPX4 protein and regulates the potential ferroptosis state of cells