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Introduction The purpose of this study is to investigate the possibility that opioids and adrenergic compounds bind directly to glutathione and to glutathione-like regions of opioid and adrenergic receptors, accounting for some of the observed negative effects of these compounds on antioxidant functions related to anesthesia and drug abuse

The rats were euthanized through cervical dislocation following blood extraction

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In all cases, the proteins were stored at 4 C until used
