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The skin was closed with the running or continuous stitch (Vycril, 3.0, Ethicon Inc., Somerville, NJ, USA)

Heres a quick checklist to decide whether you should keep going or give your scalp a break: If the tingling turns into a burning sensation, rinse the area with lukewarm water and skip the next two applications

[DOI] [PubMed] [Google Scholar] 64.Giardino L., Giuliani A., Battaglia A., Carfagna N., Aloe L., Calz L
TEM The cells were fixed overnight in a mixture of cold 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.2) and 2% paraformaldehyde in 0.1 M phosphate or cacodylate buffer (pH 7.2)
