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Prior to incubation with or without CSE, the cells were pretreated with 10 M U0126 (Cell Signaling, Beverly, MA, USA) or 0.1 mM NAC (Sigma-Aldrich Co., St Louis, MO, USA) for 2 h

Archived from the original on November 14, 2018

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Two types of combustible tobacco product use outcomes are of interest, which correspond to two key research questions: (1) ever use (i.e., starting any level of cigarette smokingeven merely a few puffs versus sustained abstinence), and (2) progression, defined as increases in smoking frequency (i.e., number of days used in past 30), intensity (i.e., cigarettes smoked per day on smoking day), and duration (i.e., length of time in which smoking behavior continues versus ceases following initiation)