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KEY WORDS: Drosophila, Heart, Tobacco, Downregulation, Nicotine, Receptors Summary: Nicotine, a substance present in tobacco, resembles insecticide compounds such as neonicotinoids, and affects the heart function in Drosophila melanogaster

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Incorporating confidence intervals in future work would enhance the statistical robustness and interpretability of the results 70 .While the highest accuracy achieved was 0.80, this may be considered modest for high-stakes clinical settings