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Warning signs of acute cholecystitis or biliary obstruction include: Persistent pain lasting more than six hours Fever and rigors (shaking chills) Jaundice (yellowing of skin and eyes) Dark urine or pale stools Severe tenderness when pressing on the right upper abdomen Charcot's triad (fever, right upper quadrant pain, and jaundice), which suggests cholangitis requiring urgent medical care It is worth noting that some gastrointestinal symptoms commonly associated with GLP-1 therapysuch as nausea, abdominal discomfort, and reduced appetitemay overlap with early gallbladder symptoms

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Then, an anti-CD34 antibody was added and incubated at 4 C overnight, after which a goat anti-rabbit IgG/Alexa Fluor 594 secondary antibody was added and incubated at 37 C for 1 h

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