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Immunocytochemistry for detection of GLP-1 Cultures of mouse intestinal cells were fixed for 10 min at room temperature in PBS containing 4% paraformaldehyde, and were then permeabilized by incubation for 10 min in PBS containing 0.25% Triton X-100

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N Engl J Med 377:644657 Wiviott SD, Raz I, Bonaca MP, Mosenzon O, Kato ET, Cahn A, Silverman MG, Zelniker TA, Kuder JF, Murphy SA, Bhatt DL, Leiter LA, McGuire DK, Wilding JPH, Ruff CT, Gause-Nilsson IAM, Fredriksson M, Johansson PA, Langkilde AM, Sabatine MS (2019) Dapagliflozin and cardiovascular outcomes in type 2 diabetes