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Regular blood sugar monitoring is recommended to assess the effectiveness and adjust dosages if necessary

Like nausea, diarrhea may improve over time with continued use of the medication

To construct the SpCas9 expression vector (referred to as the Cas9 plasmid), the Cas9 DNA fragment was amplified from hCas9 (Addgene #41815) and inserted into a CMV promoter-containing pCMV backbone vector using the In-Fusion HD cloning kit

Keep sleep and meal timing regular
