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Food is medicine Food is Medicine (FIM) programs are structural interventions in healthcare that offer food-based nutritional therapies as part of an individual's plan to manage or treat specific disease conditions and, often, social needs [222]

Clinical trials consistently show meaningful reductions in caloric intake and body weight among individuals using these therapies (Davies et al., 2021

Interestingly, chronic chemo-genetic stimulation of POA LepR-expressing neurons decreases both food intake and energy expenditure, and results in body weight loss, indicating that the reduced food intake surpasses the decreased energy expenditure [28]

C2C12 myoblasts or HEK293 cells were washed and lysed with 100 L RIPA buffer supplemented with protease inhibitors and phosphatase inhibitors (Roche)