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Footnotes This article is a PNAS Direct Submission

Obesity and pregnancy: mechanisms of short term and long term adverse consequences for mother and child

Then, GPR43 (50 nM), GPR109a (50 nM), and NC siRNA (50 nM) were delivered into the HepG2 cells (~40% confluent density) and cultured in Opti-MEM (Gibco) for 6 h, after which the transfection mixture was replaced with normal medium

Not all patients will be willing or able to take these GLP-1 drugs for many years, though, and its important that studies are done to better understand how to help individuals who want to wean off them
