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After confirming the placement with the ATII test, the internal cannula was removed and replaced with the dummy cannula

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2.4 Histological analysis For hematoxylin and eosin (H&E) staining, BAT tissues were immersed in 10% neutral-buffered formalin for fixation, followed by sequential dehydration in graded ethanol, xylene clearing, and subsequent paraffin embedding

That relative hypoglycemia produces a specific kind of tiredness, the foggy, unfocused, irritable exhaustion that makes you want to reach for sugar
