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Cells were then washed with KRBH and imaged in 3 mM glucose KRBH with images taken every 12 seconds in a 60/1.4 NA oil immersion objective with a 561 nm laser in a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices)

These effects result from delayed gastric emptying and direct effects on the gastrointestinal tract
This table shows the unadjusted analysis and adjusted analysis based on a Cox proportional hazards model with adjustment for baseline predictors of cardiorenal disease
