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Description
Cells were imaged by time-lapse microscopy using a Zeiss LSM-780 inverted confocal laser-scanning microscope with a 63/1.4 NA oil immersion objective and a 488 nm excitation wavelength and emissions at 508 and 583 nm for 2 minutes to record a baseline, followed by agonist stimulation at 100 nM for 5 minutes and 2-minute exposure to 10 M FSK + 100 M IBMX to generate a maximal response
The Beneficial Effects of GLP-1 Receptor Agonists Other than Their Anti-Diabetic and Anti-Obesity Properties
The compounded peptide industry has largely standardized around 10 mg/mL for both tirzepatide and semaglutide because it balances precision, volume, and vial economy
Second, map isoform contributions with tissue-selective readouts and test next-generation ligands for isoform bias
