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GLP-1 secretion by proteins/amino acids Protein and amino acid stimulation of GLP-1 secretion has been demonstrated in murine primary colonic L-cell cultures [260,302], in GLUTag cells [256,259], in human NCI-H716 cells [251,303] and in the isolated perfused rat ileum or colon [250,304] as well as in vivo in mice [305], rats [256,306], and humans [307,308]

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Although the use of DPP-4 inhibitors is associated with increased bone formation, their effects are more associated with mechanisms related to the suppression of bone resorption

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