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Following the two washes, the islet pellet was put on a three-level histopaque (Sigma) gradient and spun 30 min at 1,000 g , with the centrifuge brake turned off

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10.1016/j.nano.2016.08.004 [DOI] [PMC free article] [PubMed] [Google Scholar] 19.Melgar S, Karlsson L, Rehnstrm E, Karlsson A, Utkovic H, Jansson L, Michalsson E
