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The extended half-life of IGF-1 LR3 makes it particularly valuable in both medical and athletic contexts
Relative protein expression levels were quantified using ImageJ software and calculated as the ratio of the gray value of the target protein band to the internal control -actin band
Then, the slices were incubated with primary TH antibody (Sigma-Aldrich, T1299) and primary GFAP antibody (Millipore, MAB360) at 4 C overnight and then with the corresponding secondary antibody, and the results were visualized by the DAB reaction
