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ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) until the 60 min (25 mmHg), 30 min (30 mm Hg), or 30 min (40 mm Hg) reperfusion time

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NO has also been observed to nitrosylate JNK1 (blocking its autophagy-activating function) (247) and IKK, reducing AMPK phosphorylation while activating mTORC1, leading to autophagy suppression (248, 249)

Type III: This type is usually found alongside Type I
